In past decade, several efforts were made to develop green approach in the invention of stable and nanosized silver nanoparticles to overcome various shortcomings of conventional physical and chemical methods. The aim of this study was to explore facile synthesis of silver nanoparticles (AgNPs) using glycyrrhizin as a reducing and capping agent. The formation of silver nanoparticles was confirmed by sharp surface plasmon resonance at 408 nm. The produced silver nanoparticles were spherical in shape with particle size of 51.98 ± 2 nm and zeta potential of -22.9 ± 1.50 mV. Glycyrrhizin capped silver nanoparticles (GL-AgNPs) showed good stability at diverse pH, temperature and electrolyte environments. GL-AgNPs exhibited effective antimicrobial and antibiofilm activity against Escherichia coli and Staphylococcus epidermidis in vitro. Further, GL-AgNPs were evaluated for in vivo wound healing activity in Wistar rats. As GL-AgNPS displayed both antimicrobial and antibiofilm properties, they could be utilized for an effective prophylactic treatment during surgical implant practice.