Thymic stromal lymphopoietin

Thymic stromal lymphopoietin (TSLP) represents a critical factor of adaptive immune
responses while Toll-like receptors (TLRs) are key players of innate immunity
triggered by Aspergillus fumigatus (AF) in cornea. Here we stimulated
telomerase-immortalized human corneal epithelial cells (THCEs) and
telomerase-immortalized human stroma fibroblasts (THSFs) with TLR2 Ligand
zymosan, TLR4 ligand lipopolysaccharide (LPS), human recombinant TSLP or AF
hyphae for various periods, with or without the inhibition of TLR2, TLR4 or TSLP
previously. It was demonstrated that enhanced expression of TSLP was associated
with the treatment of A. fumigatus hyphae in human corneal cell lines. Stimulation of
THCEs and THSFs with zymosan or LPS induced upregulated expression of TSLP;
Inhibition of either TLR2 or TLR4 impaired the AF-induced TSLP. The Human
recombinant TSLP augmented expression of TLR2 and TLR4; Knockdown of TSLP
hindered the expression of TLRs, IL-8 and TNF-?. These findings suggested that
TSLP participated in the adaptive immune response of THCEs and THSFs triggered
by A. fumigatus, which is closely related to the function of TLRs; and the innate
immunity mediated by TLRs could be enhanced by TSLP. Thus, TSLP may serve as a
link between the innate and adaptive immune responses in corneal cell lines infected
by Aspergillus fumigatus.